Cortisol (Serum) with 24-Hour Profile

Why This Test Is Done

Your doctor may order this test if they suspect conditions like Cushing’s syndrome (too much cortisol), Addison’s disease (too little cortisol), or other hormonal imbalances. It can also help evaluate stress responses or rule out issues with your pituitary gland or adrenal glands.

What to Expect Before the Test

No special preparation is needed for the blood draw, but you may be asked to wear comfortable clothing. If you’re taking medications that affect cortisol levels, your doctor will discuss them with you. For the 24-hour urine test (if part of the profile), you’ll need to collect urine in a special container over the full day, starting after your first morning urine.

How the Test Is Done

Blood is drawn from a vein in your arm, similar to other blood tests. If measuring urine cortisol, you’ll collect all urine for 24 hours in a container provided by your lab. The test may include both serum (blood plasma) and urine samples to provide a comprehensive picture of your cortisol levels.

What the Results Mean

Results compare your cortisol levels to typical ranges found in healthy individuals. Abnormal results may indicate hormonal imbalances, stress disorders, or other medical conditions that require further evaluation by your healthcare provider.