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Cortisol, Saliva, Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cortisol, a natural steroid hormone, in your saliva over a specified period. It helps assess your body’s stress response.

Quick summary

["Saliva cortisol testing helps evaluate how your body handles stress."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
57684-3
Short code
Cortis sp11 Sal-mCnc
Synonyms
Cortis sp11 Sal-mCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Saliva cortisol testing is often used to diagnose conditions related to the adrenal glands, such as Cushing’s syndrome or Addison’s disease. It can also help determine if your body is producing too much or too little cortisol due to stress, medications, or other health issues. Unlike blood tests, saliva cortisol measures hormone levels at a specific time when you’re awake, reflecting natural daily fluctuations.

What to Expect Before the Test

No special preparation is needed. You’ll provide a saliva sample, typically collected in the morning after brushing your teeth (to avoid contamination from oral bacteria). Follow your healthcare provider’s instructions for timing and collection.

How the Test Works

A small amount of saliva is collected into a special container. The sample is then analyzed in a lab to measure cortisol levels, which are expressed in mass concentration units.

What the Results Mean

Results help identify whether cortisol levels are consistently high or low, which may indicate hormonal imbalances. Your doctor will interpret the results in relation to your symptoms and medical history.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Saliva Cortisol

Frequently asked questions

What Questions Might You Have?
Saliva cortisol testing is non-invasive and painless, but it requires following collection instructions precisely to ensure accurate results. If you’re unsure about timing or sample collection, ask your healthcare provider for guidance.
How often should I take this test?
The frequency depends on your diagnosis or symptoms. Your doctor may recommend testing over a 24-hour period or multiple morning samples to track natural fluctuations. Follow their specific instructions.
Can I take any medications before the test?
Some medications, like steroids or certain blood pressure drugs, may affect cortisol levels. Always inform your healthcare provider about all medications you’re taking before the test.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy