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Cortisol, 24-Hour Urinary Free

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cortisol, a natural steroid hormone, excreted in your urine over a 24-hour period to assess cortisol production and function of your adrenal glands.

Quick summary

["Helps evaluate adrenal gland health and potential hormone imbalances over a full day’s cycle."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
21223-3
Short code
Cortis sp1 p chal SerPl-mCnc
Synonyms
Cortis sp1 p chal SerPl-mCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if they suspect conditions like Cushing’s syndrome (overactive adrenal glands), adrenal insufficiency, or other hormonal imbalances. It can also help diagnose issues related to cortisol levels, such as stress responses or pituitary gland problems.

What to Expect Before the Test

No special preparation is needed. You’ll collect all urine for a full 24 hours, starting after you wake up and ending just before you wake up the next morning. Store the urine in a refrigerator or cool container until you deliver it to the lab.

How the Test Works

The test measures cortisol levels in your urine, which reflects how much cortisol your body produces throughout the day. Cortisol is a stress hormone that helps regulate metabolism and immune response.

What the Results Mean

Results help determine whether your cortisol levels are too high or too low. Abnormal results may indicate conditions affecting your adrenal glands, pituitary gland, or stress responses.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: Cortisol is essential for managing stress, immune function, and metabolism. Elevated or low levels can signal underlying health issues, including hormonal disorders, infections, or chronic stress.

Short name

24-Hour Urine Cortisol

Frequently asked questions

What causes abnormal cortisol levels in urine?
Abnormal results may be due to conditions like Cushing’s syndrome (high cortisol), adrenal insufficiency (low cortisol), or prolonged stress. Other factors could include medications, certain tumors, or pituitary disorders.
How long does it take to get results?
Results are typically available within 1 to 2 weeks after the lab processes your sample, depending on the lab’s workflow.
Can I take medications before this test?
Yes, but inform your doctor about all medications, including steroids or supplements, as they may affect cortisol levels. Follow their guidance on timing.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy