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Cortisol, Saliva, Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cortisol, a natural steroid hormone, in your saliva. It helps assess your body’s stress response and adrenal gland function.

Quick summary

["Saliva cortisol testing helps evaluate how your body handles stress and adrenal health."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
57685-0
Short code
Cortis sp2 Sal-mCnc
Synonyms
Cortis sp2 Sal-mCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Saliva cortisol testing is often used to diagnose conditions like Cushing’s syndrome (overactive adrenal glands) or Addison’s disease (underactive adrenal glands). It can also help monitor stress levels or rule out hormonal imbalances. Unlike blood tests, saliva samples are easier to collect and don’t require fasting.

How the Test Works

You’ll provide a saliva sample (like spitting into a tube) at a specific time—often early morning for baseline levels. The test measures cortisol concentration, which naturally peaks in the morning and declines later in the day.

Sample Collection

Saliva is collected using a special collection device (like a cotton swab or tube) placed under your tongue. You’ll avoid eating, drinking (except water), or brushing your teeth for 30 minutes before collecting the sample.

What to Expect Before the Test

No special preparation is needed beyond avoiding certain foods/drinks before collection. If you’re taking medications (e.g., steroids), your doctor may advise timing adjustments.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Elevated cortisol in saliva may indicate chronic stress, Cushing’s syndrome, or other adrenal disorders. Low levels could suggest Addison’s disease or pituitary issues. Results help guide treatment for hormonal imbalances or stress management plans.

Short name

Saliva Cortisol

Frequently asked questions

What Does a High Cortisol Level Mean?
A high cortisol level may signal chronic stress, Cushing’s syndrome (excess cortisol production), or long-term use of certain medications. Your doctor will review your symptoms and other test results to determine the cause.
What Does a Low Cortisol Level Mean?
Low cortisol could indicate Addison’s disease (adrenal insufficiency), pituitary problems, or other hormonal disorders. This may require further testing or treatment to restore balance.
Can Stress Affect Saliva Cortisol Results?
Yes, stress can temporarily raise cortisol levels. If you’re managing stress (e.g., through lifestyle changes), your results may reflect that. Your doctor may recommend retesting after a period of stability.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy