Cortisol, Saliva, 24-Hour Urine Free Metabolite (Total Cortisol Metabolite)
This test measures the total amount of cortisol metabolites found in your saliva and urine over 24 hours. It helps assess your body’s cortisol production and adrenal function.
["This test helps evaluate long-term cortisol levels, useful for diagnosing conditions like Cushing’s syndrome or adrenal insufficiency."]
Key facts at a glance
- LOINC
- 58673-5
- Short code
- Cortis sp4 p chal Sal-sCnc
- Synonyms
- Cortis sp4 p chal Sal-sCnc
- Category
- hormone
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why This Test Is Done
Your doctor may recommend this test if they suspect hormonal imbalances related to cortisol, such as excessive cortisol production (Cushing’s syndrome) or adrenal gland issues. It can also be used to monitor treatment responses in certain conditions.
How the Test Works
You’ll collect saliva samples throughout the day and provide a 24-hour urine sample. These samples are analyzed to measure cortisol metabolites, which reflect overall cortisol exposure over time.
Sample Collection Process
Saliva samples are typically collected using cotton swabs or special collection devices placed under your tongue. Urine is collected in a container over a full 24-hour period. Follow your healthcare provider’s instructions precisely for accurate results.
What to Expect Before the Test
No special preparation is usually needed, but avoid caffeine or certain medications that might affect cortisol levels before testing. If you’re on long-term medications, discuss this with your doctor.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Elevated levels may indicate Cushing’s syndrome, a condition caused by prolonged high cortisol. Low levels could suggest adrenal insufficiency or other hormonal disorders. This test helps diagnose and monitor conditions affecting cortisol metabolism and adrenal function.
Short name
Saliva & Urine Cortisol Metabolite
Frequently asked questions
How long does it take to get results?
What does a high or low result mean?
Can I take medications before or after this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy