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Cortisol, Saliva, Free, Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cortisol, a natural steroid hormone, in your saliva. Cortisol helps regulate metabolism, immune response, and stress reactions.

Quick summary

["Saliva cortisol testing helps assess your body’s stress and hormone balance."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
57689-2
Short code
Cortis sp4 Sal-mCnc
Synonyms
Cortis sp4 Sal-mCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Saliva cortisol testing may be recommended if you have symptoms of stress, adrenal gland issues, or conditions like Cushing’s syndrome or Addison’s disease. It can also help monitor treatment for these conditions. Unlike blood tests, saliva cortisol reflects recent hormone levels without the need for fasting.

What to Expect Before the Test

No special preparation is needed. Collect a saliva sample by gently chewing a cotton swab for about 5 minutes. Spit into a provided container. Avoid eating, drinking (except water), smoking, or brushing your teeth for 30 minutes before collecting the sample.

How the Test Works

A healthcare provider will send your saliva sample to a lab, where it is analyzed using a sensitive immunoassay method to measure cortisol levels. This test captures cortisol released during the day, reflecting your body’s natural rhythm.

What the Results Mean

Results are compared to typical ranges for cortisol in saliva, but exact values may vary by lab. High levels may indicate overproduction of cortisol, while low levels could suggest adrenal insufficiency or other hormonal imbalances. Your doctor will interpret results in the context of your symptoms and medical history.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Saliva Cortisol

Frequently asked questions

What does cortisol do in my body?
Cortisol is produced by your adrenal glands and plays a key role in managing stress, immune function, metabolism, and blood pressure. It peaks in the morning and declines throughout the day. Abnormal cortisol levels can affect energy, weight, mood, and overall health.
How can I prepare for follow-up care if my results are abnormal?
If your saliva cortisol results are outside the expected range, your doctor may order additional tests, such as a blood cortisol test or a 24-hour urine cortisol test, to get a clearer picture. They may also review your symptoms, medical history, and other medications to determine the next steps.
Can I take anything to lower my cortisol levels before the test?
Avoiding stress, caffeine, or alcohol for 24 hours before the test can help ensure more accurate results. However, do not take any medications (including over-the-counter supplements) without consulting your doctor first, as they may affect cortisol levels.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy