Skip to content

Cortisol in Serum (Mass Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cortisol, a natural steroid hormone produced by your adrenal glands, in your blood serum.

Quick summary

["Cortisol helps regulate metabolism, immune response, and stress reactions."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
50457-1
Short code
Cortis sp5 SerPl-mCnc
Synonyms
Cortis sp5 SerPl-mCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Might This Test Be Ordered?

Your healthcare provider may recommend this test if you have symptoms like unexplained weight changes, fatigue, high blood pressure, or signs of adrenal gland issues. It could also be used to diagnose conditions such as Cushing’s syndrome, adrenal insufficiency, or to monitor treatment for hormone-related disorders.

What Does the Test Involve?

A blood sample will be taken from a vein in your arm. The sample is then processed to separate serum, which is analyzed for cortisol levels using a sensitive laboratory method.

What to Expect Before or After the Test?

No special preparation is usually needed, but you may be advised to avoid certain medications or activities depending on your specific situation. After the test, you might experience mild discomfort or bruising at the injection site, which typically resolves quickly.

Common Conditions Monitored or Diagnosed With This Test

Cortisol levels help diagnose or monitor conditions like adrenal gland disorders (such as Addison’s disease or Cushing’s syndrome), evaluate stress responses, or assess the effectiveness of hormone replacement therapy.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Serum Cortisol

Frequently asked questions

What Are Normal Results?
Normal cortisol levels can vary depending on the time of day and individual factors. Your results will be compared to your healthcare provider’s reference ranges specific to your situation.
How Long Will It Take to Get Results?
Results are typically available within a few days to a week, depending on your lab’s processing schedule. Follow up with your healthcare provider to discuss the findings.
Can Cortisol Levels Change Due to Diet or Medications?
Yes, certain foods, medications, or supplements can affect cortisol levels. If you’re taking medications like steroids or have dietary restrictions, inform your healthcare provider before the test.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy