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Cortisol, Saliva, Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cortisol, a natural steroid hormone, in your saliva. It helps assess your body’s stress response and cortisol levels over time.

Quick summary

["Saliva cortisol testing can help track stress-related hormone fluctuations."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
57693-4
Short code
Cortis sp6 Sal-mCnc
Synonyms
Cortis sp6 Sal-mCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Doctors may order saliva cortisol tests to evaluate conditions like Cushing’s syndrome (overactive cortisol production), adrenal insufficiency (underactive cortisol production), or chronic stress. It can also help diagnose pituitary disorders or monitor treatment responses in hormone-related conditions.

What to Expect Before the Test

No special preparation is needed. You’ll provide a saliva sample, usually collected in the morning when cortisol levels are highest. Avoid caffeine, smoking, or strenuous activity for 30 minutes before sampling.

How the Test Works

A small amount of saliva is collected using a cotton swab or saliva collection device. The test measures cortisol concentration directly from the sample, often using immunoassay methods.

What the Results Mean

Results help determine if cortisol levels are consistently high or low, aiding diagnosis or monitoring. Interpretation depends on clinical context, such as timing (e.g., morning vs. evening samples) and comparison to baseline or reference ranges.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Saliva Cortisol

Frequently asked questions

What are the normal ranges for this test?
Normal ranges vary by lab and testing method. Your doctor will interpret results in relation to your symptoms, medical history, and other tests. For example, elevated cortisol may suggest Cushing’s syndrome, while persistently low levels could indicate adrenal insufficiency.
Can I take anything before my saliva cortisol test?
Avoid caffeine, nicotine, or alcohol for at least 30 minutes before testing. Some medications (e.g., steroids) may also affect results, so discuss them with your healthcare provider.
How often will I need this test?
Frequency depends on your diagnosis or treatment plan. Your doctor may recommend periodic testing (e.g., monthly or annually) to monitor cortisol levels over time, especially if managing stress-related conditions or hormone therapies.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy