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Cortisol, Saliva, Free, Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of free cortisol present in your saliva, which reflects your body’s natural cortisol levels over a 24-hour period.

Quick summary

["Saliva cortisol helps assess your body’s stress response and adrenal function."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
57694-2
Short code
Cortis sp7 Sal-mCnc
Synonyms
Cortis sp7 Sal-mCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order a saliva cortisol test to evaluate conditions related to stress, adrenal gland function, or hormonal imbalances. It can also help diagnose or monitor Cushing’s syndrome, adrenal insufficiency, or other hormonal disorders.

What to Expect Before the Test

No special preparation is needed. You’ll provide a saliva sample, typically collected over a 24-hour period (morning and evening samples). Follow your provider’s instructions for timing and collection.

What Happens During the Test?

You’ll collect saliva samples in a sterile container at specified times. The samples are then sent to a lab for analysis using a high-performance liquid chromatography (HPLC) method to measure cortisol levels accurately.

What Are the Results?

Results are compared to reference ranges for cortisol in saliva, though exact values may vary by lab. Your provider will interpret the results in the context of your symptoms and medical history.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Saliva Cortisol

Frequently asked questions

What Does It Mean If My Results Are Abnormal?
High cortisol levels in saliva may indicate conditions like Cushing’s syndrome, where the body produces too much cortisol. Low levels could suggest adrenal insufficiency or other stress-related disorders. Your provider will discuss findings with you.
How Often Should I Get This Test?
This test is often used as part of a diagnostic evaluation rather than a routine screening. Your provider will determine how often to repeat it based on your symptoms and medical needs.
Can I Take Medications Before the Test?
Some medications, like steroids or certain stress-related drugs, may affect cortisol levels. Always inform your provider about all medications or supplements you’re taking before the test.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy