Skip to content

Cortisol, Saliva, 24-Hour Average (Qualitative or Quantitative Measurement)

Published: August 9, 2026Updated: August 16, 2026

This test measures cortisol levels in your saliva over a 24-hour period to assess your body’s cortisol production and help diagnose conditions related to stress or adrenal function.

Quick summary

["Helps evaluate cortisol levels linked to stress, adrenal disorders, or circadian rhythm issues."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
58696-6
Short code
Cortis sp8 p chal Sal-sCnc
Synonyms
Cortis sp8 p chal Sal-sCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may recommend this test if they suspect conditions like Cushing’s syndrome (too much cortisol), adrenal insufficiency (too little cortisol), or irregular stress responses. It can also help confirm whether cortisol follows normal daily patterns.

How the Test Works

You’ll collect saliva samples throughout the day, typically at consistent intervals, into a special container. The samples are then analyzed to determine the average cortisol concentration over 24 hours.

Sample Collection Process

You’ll receive instructions on how to collect saliva samples at home, usually by spitting into a tube at set times. Avoid eating, drinking, or brushing your teeth before collection to ensure accurate results.

Preparation Tips

Follow your doctor’s instructions carefully. Avoid caffeine, nicotine, or alcohol before collection, as they can affect cortisol levels. Wear comfortable clothing for easy access to your mouth.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Abnormally high or low cortisol levels can indicate underlying health issues, such as hormonal imbalances, chronic stress, or autoimmune disorders. This test helps identify whether cortisol production is disrupted and guides treatment decisions.

Short name

Saliva Cortisol Profile

Frequently asked questions

How long will I need to collect saliva samples?
You’ll need to collect saliva samples for approximately 24 hours, usually at consistent intervals (e.g., every 2–4 hours). Your healthcare provider will provide specific instructions.
What should I do if I miss a sample collection time?
If you miss a scheduled collection, contact your lab or doctor immediately to reschedule. Missing samples can affect the accuracy of your results, so follow their guidance to minimize disruption.
Will I need to fast before this test?
No fasting is required for this test, but avoid eating, drinking (except water), brushing your teeth, or using mouthwash for about 30 minutes before each collection to prevent contamination.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy