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Cortisol, Saliva, Free, Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cortisol, a natural steroid hormone, in your saliva. Cortisol helps regulate stress responses and other bodily functions.

Quick summary

["Assesses your body’s cortisol levels naturally through saliva, useful for diagnosing stress-related conditions."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
57695-9
Short code
Cortis sp8 Sal-mCnc
Synonyms
Cortis sp8 Sal-mCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Saliva cortisol testing is often used to evaluate your body’s cortisol production over time, especially in conditions like Cushing’s syndrome or adrenal insufficiency. Unlike blood tests, saliva cortisol reflects free (unbound) cortisol levels, which can be more accurate for assessing stress-related hormone fluctuations.

What to Expect Before the Test

No special preparation is needed. You’ll provide a saliva sample, typically collected in the morning when cortisol levels are highest. Avoid caffeine, nicotine, or alcohol for 30–60 minutes before collecting your sample to ensure accurate results.

How the Test Works

A small amount of saliva is collected into a container. The sample is then analyzed to measure cortisol concentration using a sensitive immunoassay method, which detects the hormone’s presence without interference from other substances.

What the Results Mean

Results help doctors determine if your cortisol levels are consistently too high or too low, which can indicate hormonal imbalances or stress-related disorders. Your doctor will compare these results with your medical history and other tests.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Saliva Cortisol

Frequently asked questions

How long does it take to get my saliva cortisol results?
Results are typically available within 1–3 business days, depending on your lab’s processing schedule. Follow-up with your healthcare provider to discuss the findings and next steps.
Can I take anything before my saliva cortisol test?
Avoid caffeine, nicotine, or alcohol for 30–60 minutes before collecting your saliva sample to prevent false results. Stick to water and avoid brushing your teeth during this time.
Is this test painful or uncomfortable?
No, this test is completely painless. You simply collect saliva into a small container, similar to spitting into a cup, and submit it to the lab for analysis.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy