Cotinine in urine (24-hour collection)

Why Is This Test Done?

Your healthcare provider may order this test to monitor nicotine use during smoking cessation programs, evaluate compliance with nicotine replacement therapy, or assess exposure to secondhand smoke in certain settings. It’s particularly useful for tracking progress in quitting smoking because cotinine persists in the body longer than nicotine itself.

What to Expect Before the Test

For a 24-hour urine cotinine test, you’ll collect all urine produced in a sealed container over a full day. Follow your provider’s instructions carefully to ensure accurate results. Avoid certain medications or substances that might interfere with the test, such as some over-the-counter cold or allergy medications.

What Happens During the Test?

No special preparation is needed beyond collecting urine in a sterile container over 24 hours. The sample is then sent to a lab for analysis using a sensitive immunoassay method to detect cotinine levels.

What Do the Results Mean?

Results are typically reported as the total amount of cotinine excreted in the urine over 24 hours. Higher levels generally indicate recent nicotine exposure, while lower levels suggest reduced or no exposure. Your healthcare provider will interpret the results in the context of your medical history and treatment plan.