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Cotinine (24-hour urine)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cotinine, a metabolite of nicotine, in your urine over a 24-hour period. It helps assess exposure to tobacco smoke or nicotine use.

Quick summary

["This test checks for recent nicotine exposure, often used in smoking cessation monitoring."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
109643-7
Short code
Cotinine Msmt Sal
Synonyms
Cotinine Msmt Sal
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you suspect exposure to tobacco smoke, nicotine, or if you’re being monitored for smoking cessation. It can also help determine if someone else’s smoking affects you (e.g., secondhand exposure).

What to Expect Before the Test

You’ll collect all urine produced in a 24-hour period in a special container. Drink plenty of fluids to ensure full collection. Avoid smoking or vaping before testing, as it may affect results.

How the Test Works

The collected urine is analyzed to measure cotinine levels. This metabolite is stable in urine and reflects recent nicotine exposure.

Possible Results and Interpretation

Higher levels may indicate recent tobacco or nicotine use, while lower levels suggest reduced exposure. Your provider will interpret results based on clinical context, such as your medical history or symptoms.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24-Hour Urine Cotinine

Frequently asked questions

How long does it take to get results?
Results are typically available within 1–3 business days, depending on lab processing times.
Can I drink coffee or other beverages while collecting urine?
Yes, but avoid caffeine-rich drinks if your provider specifies it. Drink water to help urine flow, but ensure you collect all urine produced in the 24-hour period.
Will I need to stop smoking before this test?
Yes, avoid smoking or vaping for at least 24 hours before testing to get an accurate measurement of nicotine exposure.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy