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Cotinine in Saliva (Mass Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cotinine, a metabolite of nicotine, in your saliva. It helps assess recent nicotine exposure, often used in monitoring smoking cessation programs.

Quick summary

["This test checks for nicotine use by detecting its breakdown product in saliva."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
74371-6
Short code
Cotinine Sal Cfm-mCnc
Synonyms
Cotinine Sal Cfm-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
confirm
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you're trying to quit smoking or are being monitored for nicotine use. It provides a direct measure of nicotine exposure over the past few hours, unlike blood tests that show longer-term exposure.

How the Test Works

A small saliva sample is collected, typically from the mouth, and analyzed using a sensitive laboratory method to measure cotinine levels. This test is often used in research or clinical settings to track progress in smoking cessation.

What to Expect Before the Test

No special preparation is needed. You may brush your teeth or rinse your mouth with water before collecting the sample, but avoid eating, drinking, or smoking immediately before testing to ensure accurate results.

What the Results Mean

Results are interpreted by your healthcare provider based on your individual circumstances. Higher levels may indicate recent nicotine use, while lower levels suggest reduced exposure or successful abstinence.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Saliva Cotinine

Frequently asked questions

What does a high level of cotinine in saliva indicate?
A high cotinine level in saliva strongly suggests recent nicotine exposure, often from smoking or vaping. This can help confirm whether you’ve been using nicotine products as monitored in a program.
How often should I get this test if I’m quitting smoking?
The frequency depends on your treatment plan. Your healthcare provider may recommend testing at specific intervals (e.g., weekly or monthly) to track progress and adjust support as needed.
Can diet or oral hygiene affect cotinine test results?
While brushing your teeth or rinsing your mouth before testing can help avoid contamination, diet or oral hygiene itself does not directly impact cotinine levels. Avoid eating or drinking for about 30 minutes before collection.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy