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Cotinine in Saliva (24-Hour Collection)

Published: August 9, 2026Updated: August 16, 2026

This test measures cotinine, a metabolite of nicotine, in your saliva over a 24-hour period. It helps assess recent exposure to tobacco smoke or nicotine.

Quick summary

["This test helps track nicotine exposure and supports decisions about smoking cessation."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
74370-8
Short code
Cotinine Sal Ql Cfm
Synonyms
Cotinine Sal Ql Cfm
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
confirm
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test if you are trying to quit smoking, monitoring your progress, or evaluating exposure to secondhand smoke. It can also be used in research or workplace safety settings to measure nicotine intake.

What Does the Test Involve?

You’ll collect saliva samples throughout the day in a provided container. The test measures the total cotinine level accumulated in your saliva over 24 hours. No fasting is required.

What to Expect Before the Test

No special preparation is needed, but avoid chewing tobacco or using nicotine-containing products during the collection period. If you smoke, use the provided collection kit as directed.

What Are the Results Typically Used For?

Results help determine how much nicotine you’ve been exposed to, track progress in quitting smoking, or assess compliance with nicotine reduction programs. Lower levels suggest reduced exposure.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: This test is primarily used in clinical and research settings to monitor nicotine dependence, evaluate smoking cessation efforts, and assess environmental tobacco exposure. Cotinine levels are more reliable than urine tests for detecting recent nicotine use due to its longer half-life in saliva.

Short name

Saliva Cotinine (24h)

Frequently asked questions

How long does it take to get my results?
Results are typically reported within 3 to 10 business days, depending on lab processing times. Follow-up with your healthcare provider to discuss your findings.
Can I drink coffee or eat before collecting saliva samples?
Yes, you may drink water or eat normally, as saliva collection does not require fasting. Avoid chewing tobacco or using nicotine gum/patches during the 24-hour period.
What if I miss some saliva samples during the collection period?
If you miss samples, collect them as soon as possible. If you miss more than a few hours, notify your lab or provider to adjust the collection period or retest.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy