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Cotinine in Urine (Mass Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cotinine, a metabolite of nicotine, in your urine. It helps assess recent exposure to tobacco smoke.

Quick summary

["Cotinine helps track nicotine use, even if you don’t smoke yourself."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
9372-4
Short code
Cotinine Spec-mCnc
Synonyms
Cotinine Spec-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you suspect exposure to secondhand smoke, especially in children or individuals with respiratory issues. It can also be used in research or workplace safety settings to monitor nicotine exposure.

What to Expect Before the Test

No special preparation is needed. You’ll provide a urine sample, typically collected in the morning. Avoid smoking or chewing tobacco for at least 24 hours before testing if instructed.

What the Results Mean

Higher levels may indicate recent exposure to nicotine, such as from smoking or vaping. Lower levels could mean reduced exposure or recent abstinence. Results are interpreted by your provider based on your situation.

Common Reasons for Testing

This test is often used in studies, workplace safety assessments, or to evaluate secondhand smoke exposure in non-smokers.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Urine Cotinine

Frequently asked questions

What does a positive result mean?
A positive result suggests recent nicotine exposure, which could come from smoking, vaping, or secondhand smoke. Your provider will discuss next steps based on your health history and symptoms.
Can diet or other medications affect this test?
Dietary factors or certain medications may not significantly interfere with cotinine levels, but smoking or nicotine-containing products should be avoided for at least 24 hours before testing to ensure accurate results.
How long do I need to wait for results?
Results are typically available within a few days to a week, depending on lab processing times. Your provider will contact you once they’re ready to discuss them.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy