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Cotinine in Urine (24-hour collection)

Published: August 9, 2026Updated: August 16, 2026

This test measures the presence of cotinine, a metabolite of nicotine, in your urine over a 24-hour period. It helps assess exposure to tobacco smoke or nicotine.

Quick summary

["This test checks for recent nicotine exposure, often used to monitor smoking habits or detect secondhand smoke exposure."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
60299-5
Short code
Cotinine Spec-sCnc
Synonyms
Cotinine Spec-sCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test if you suspect exposure to tobacco smoke, nicotine, or if you’re part of a study or program evaluating smoking cessation. It can also help determine compliance with nicotine replacement therapy.

What to Expect Before the Test

You’ll collect all urine produced in a 24-hour period in a special container. Drink plenty of fluids to ensure full collection. Avoid smoking or using nicotine products before testing.

What to Expect During the Test

No special preparation is needed beyond the 24-hour urine collection. The sample is sent to a lab for analysis.

What the Results Mean

Higher levels of cotinine suggest recent nicotine exposure, while lower levels may indicate reduced or no exposure. Results are interpreted by your healthcare provider based on your clinical situation.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24-Hour Urine Cotinine

Frequently asked questions

How long does it take to get my results?
Results are typically available within 1 to 3 business days, depending on your lab’s processing time. Your healthcare provider will discuss the findings with you.
Can I drink alcohol before this test?
Alcohol consumption does not typically affect cotinine levels, so you can drink as usual. However, avoid smoking or nicotine products during the 24-hour collection period.
What if I miss any urine during the collection?
If you miss urine, you’ll need to restart the 24-hour collection. Ensure you drink fluids to help maintain urine flow and complete the sample properly.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy