Cotinine (Mass Concentration) in Urine

Why Is This Test Done?

Your healthcare provider may order this test if you suspect exposure to tobacco smoke, nicotine, or if you’re part of a study evaluating nicotine use. It’s also sometimes used in workplace safety assessments or to monitor compliance with nicotine-free environments.

What Does a Positive Result Mean?

A positive result indicates recent exposure to nicotine or tobacco products. The presence of cotinine suggests you’ve been exposed within the last few days, though it doesn’t confirm direct smoking—it can also come from secondhand smoke or nicotine-containing products.

What Should You Expect During the Test?

You’ll provide a urine sample, typically collected in a sterile container. The test is quick and painless, often done as part of a routine lab visit. No special preparation is needed, but avoid drinking excessive fluids before the test if instructed.

What Are Common Causes of Abnormal Results?

Cotinine levels can rise from smoking cigarettes, vaping, chewing tobacco, or using nicotine patches/gum. Secondhand smoke exposure can also contribute. Rarely, certain medications or medical conditions may affect results, but this is uncommon.