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Cotinine (24-hour urine)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cotinine, a breakdown product of nicotine, in your urine over a 24-hour period. It helps assess recent exposure to tobacco smoke.

Quick summary

["Cotinine urine tests track nicotine use for smoking cessation support."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
12293-7
Short code
Cotinine Ur Ql
Synonyms
Cotinine Ur Ql
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What Is Cotinine?

Cotinine is a metabolite (byproduct) of nicotine, the primary active compound in tobacco. Your body breaks down nicotine into cotinine, which remains in your system for several days. This test detects cotinine levels in your urine to gauge how much nicotine you’ve been exposed to recently.

Why Is This Test Done?

Healthcare providers use cotinine tests to monitor tobacco use, especially in children, adolescents, or individuals trying to quit smoking. It’s often used in research, workplace screenings, or as part of smoking cessation programs to verify compliance with nicotine-free environments.

How Is the Test Performed?

You’ll collect a 24-hour urine sample by collecting all urine produced in a single day, typically from one morning to the next. The sample is sealed and returned to the lab for testing. The test measures cotinine concentration in micrograms per liter (mcg/L) or milligrams per liter (mg/L).

What Does the Result Mean?

Results are interpreted based on the measured cotinine concentration. Low levels may indicate minimal or no tobacco exposure, while higher levels suggest recent smoking or secondhand exposure. Your healthcare provider will discuss the results with you in context of your health goals.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

How long does it take to process this test?
Processing times vary by lab, but results are typically available within 1–3 business days after the sample is received. Follow-up with your healthcare provider is recommended to review results and next steps.
Can I drink coffee or other beverages before the test?
No, avoid caffeine-containing drinks (like coffee, tea, or soda) for 24 hours before collecting your urine sample, as caffeine can affect cotinine measurement accuracy.
What if I smoke or use tobacco products during the 24-hour period?
If you smoke or use tobacco products during the collection period, your cotinine levels will reflect that exposure. If you’re trying to quit, discuss this with your healthcare provider to ensure accurate monitoring of your progress.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy