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Cotinine in Urine

Published: August 9, 2026Updated: August 16, 2026

This test measures the presence of cotinine, a metabolite of nicotine, in your urine. It helps assess recent exposure to tobacco smoke or nicotine.

Quick summary

["This test helps track nicotine use over the past few days."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
33796-4
Short code
Cotinine Ur-sCnc
Synonyms
Cotinine Ur-sCnc
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test if you suspect recent exposure to tobacco smoke or nicotine, such as secondhand smoke, or if you’re being monitored for nicotine dependence. It can also be used in workplace safety programs or during treatment for smoking cessation.

What Does a Positive Result Mean?

A positive result indicates detectable levels of cotinine, suggesting recent nicotine exposure. The exact amount can help estimate how much nicotine you’ve been exposed to, but it’s not a direct measure of smoking frequency alone.

How Is the Test Performed?

You’ll provide a urine sample, typically collected in a sterile container. The sample is then sent to a lab for analysis using a specialized method to measure cotinine concentration.

What Are Common Uses?

This test is commonly used in clinical settings to monitor compliance with smoking cessation programs, assess secondhand smoke exposure, or evaluate nicotine dependence in medical research or workplace health programs.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Urine Cotinine

Frequently asked questions

How long does it take to get results?
Results are typically available within 1 to 3 business days, depending on lab processing times. Your healthcare provider will contact you with the outcome once it’s ready.
Can I drink before giving a urine sample?
Yes, you can drink water before giving your urine sample, but avoid excessive fluid intake to ensure a concentrated sample. Follow any specific instructions your lab provides.
Does this test detect other substances besides nicotine?
No, this test specifically measures cotinine, the metabolite of nicotine. It does not detect other substances like alcohol, drugs, or other chemicals in your urine.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy