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Cotinine in urine (mass concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cotinine, a breakdown product of nicotine, in your urine. It helps assess recent exposure to tobacco smoke or nicotine.

Quick summary

["This test checks for nicotine exposure over the past 24 hours."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
72786-7
Short code
Cotinine Ur Scn-mCnc
Synonyms
Cotinine Ur Scn-mCnc
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
screen
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why is this test done?

Your healthcare provider may order this test to evaluate exposure to tobacco smoke or nicotine, especially if you or someone in your household smokes. It can also be used in medical research or workplace safety assessments.

What does the result mean?

Higher levels of cotinine in urine typically indicate recent exposure to nicotine. However, interpretation depends on clinical context, such as smoking status or exposure history. Your doctor will discuss the results with you.

How is the test performed?

A urine sample is collected in a sterile container. The sample is then sent to a lab where it is analyzed using a sensitive chemical method to measure cotinine levels.

What should I expect before the test?

No special preparation is usually needed, but you may be asked to avoid certain medications or supplements that could interfere with the test results. Follow your healthcare provider’s instructions.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Cotinine levels in urine are useful for detecting recent nicotine exposure because they reflect recent smoking or vaping activity. It is more reliable than measuring nicotine alone, as cotinine persists longer in the body. This test can be particularly valuable in cases of suspected secondhand smoke exposure or in monitoring compliance with smoking cessation programs.

Short name

Urine Cotinine

Frequently asked questions

How long does it take to get the results?
Results are typically available within 1 to 3 business days, depending on the lab’s processing schedule. Your healthcare provider will contact you once the results are ready.
Can I drink alcohol before the test?
Alcohol itself does not affect cotinine levels, but if you are concerned about potential interference from medications or supplements, check with your healthcare provider before the test.
Is there anything I should avoid doing after the test?
There are no specific restrictions after the test, but if you have any questions about your medication or lifestyle changes, discuss them with your doctor.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy