Skip to content

Protein Electrophoresis with Bromocresol Purple Staining (Serum/Plasma)

Published: August 9, 2026Updated: August 16, 2026

This test measures the distribution of proteins in your blood using a technique called electrophoresis. It helps identify patterns of protein separation that may indicate conditions affecting protein production or breakdown.

Quick summary

["This test helps doctors diagnose and monitor conditions linked to abnormal protein levels in your blood."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
25579-4
Short code
C peptide sp6 p chal SerPl-sCnc
Synonyms
C peptide sp6 p chal SerPl-sCnc
Category
coagulation
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
0.8
Ref high
1.2
Critical range
— – 5 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
85610
SNOMED
396252006
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

Electrophoresis separates blood proteins based on their size and electrical charge. Bromocresol Purple staining highlights specific protein bands, showing how proteins like albumin, globulins, and other fractions are distributed. This can reveal imbalances that might suggest conditions like liver disease, kidney disease, or certain cancers.

How the Test Works

A small blood sample is taken from your arm and processed to separate proteins. The sample is placed in an electric field, causing proteins to migrate toward the opposite charge. After staining, the pattern of bands is analyzed to assess protein composition.

Common Uses

Doctors may order this test to investigate symptoms like swelling, fatigue, or unexplained weight changes. It’s also used to monitor conditions like multiple myeloma, liver disease, or chronic kidney disease, where protein patterns can change over time.

When You Might Need This Test

You might need this test if you have symptoms like unexplained swelling (edema), frequent infections, or if your doctor suspects a condition affecting protein production or breakdown in your body.

Reference range

INR: 0.8-1.2 (warfarin: 2.0-3.0)N/A

Specimen: serum

Inform your healthcare provider if you are taking blood thinners. No special preparation required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Bromocresol Purple Protein Electrophoresis

Frequently asked questions

Why might my doctor want to see these protein patterns?
Your doctor may look for abnormal bands or shifts in protein distribution to diagnose or track conditions like liver disease (e.g., cirrhosis), kidney disease, or certain blood disorders. For example, high globulin levels might suggest an immune system issue, while low albumin could indicate liver or kidney problems.
How long will results take?
Results are typically available within 24 to 48 hours, depending on lab processing times. Your doctor will discuss the findings with you during your follow-up visit.
What does it mean if my protein levels are abnormal?
Abnormal results may indicate an underlying health issue, but your doctor will interpret them in the context of your symptoms and medical history. Some conditions, like multiple myeloma, show distinct protein patterns that help guide treatment decisions.
  1. 1
  2. 2
  3. 3

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy