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Total Protein with Electrophoresis Separation (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures the overall protein content in your blood and separates different protein types using gel electrophoresis. It helps identify abnormal protein patterns, which may indicate conditions like chronic inflammation, infections, or certain blood disorders.

Quick summary

["This test helps doctors diagnose and monitor conditions affecting blood protein balance."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
95929-6
Short code
CT Heel for BMD
Synonyms
CT Heel for BMD
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ct
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Does This Test Measure?

The test first checks the total protein level in your serum, which includes proteins like albumin and globulins. Then, electrophoresis separates these proteins into distinct bands based on size and charge. This separation helps identify specific protein abnormalities.

Why Might You Need This Test?

Your doctor may order this test if you have symptoms like swelling, unexplained weight loss, fatigue, or if they suspect conditions like liver disease, kidney problems, multiple myeloma, or chronic infections.

Preparation and Sample Collection

No special preparation is needed, but you may be asked to fast for 8–12 hours before the blood draw if albumin is also measured. A healthcare provider will collect a blood sample from a vein in your arm.

Common Conditions Monitored

This test can help diagnose or monitor conditions such as hypoalbuminemia (low albumin), protein-losing disorders, or abnormal protein synthesis, such as in multiple myeloma or amyloidosis.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total Protein Electrophoresis

Frequently asked questions

How Long Does It Take to Get Results?
Results are typically available within 24–48 hours, depending on lab processing times. Follow-up with your doctor to discuss findings and next steps.
What Are Normal Results?
Normal results vary by lab, but a total protein test often includes a range for albumin and globulin fractions. Abnormal patterns may suggest underlying health concerns requiring further evaluation.
Can I Eat or Drink Before the Test?
If fasting is required for albumin measurement, avoid food and liquids (except water) for 8–12 hours before your blood draw. Otherwise, you can eat and drink normally.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy