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Hemoglobin A1c (HbA1c) with 24-hour urine collection for total 24-hour urinary excretion of free cortisol

Published: August 9, 2026Updated: August 16, 2026

This test measures your average blood sugar levels over the past 2–3 months and checks how much cortisol you excrete in a 24-hour urine sample.

Quick summary

["Helps track long-term blood sugar control and assess cortisol balance in your urine."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
77448-9
Short code
CT Hind+Mid ft
Synonyms
CT Hind+Mid ft
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ct
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Matters for Blood Sugar Control

HbA1c reflects the percentage of your red blood cells coated with hemoglobin that has been linked to glucose over time. It’s a key indicator of how well your diabetes or prediabetes is managed, giving a snapshot of your average blood sugar levels over months rather than just recent days.

How HbA1c Works in Your Body

HbA1c forms when glucose attaches to hemoglobin in your red blood cells. Since red blood cells live for about 3 months, this test provides a long-term view of your blood sugar trends, which can help prevent complications like nerve damage or heart disease.

Understanding Cortisol in Urine

Cortisol is a stress hormone produced by your adrenal glands. Measuring its excretion in urine helps assess how your body handles stress and hormone regulation, which can be important for conditions like Cushing’s syndrome or adrenal dysfunction.

What to Expect Before the Test

For the HbA1c, no special preparation is needed. For the 24-hour urine cortisol test, you’ll collect all urine for a full day, starting and ending at the same time each day. Drink plenty of water but avoid caffeine or medications that affect cortisol levels before testing.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: HbA1c and Cortisol Together

Short name

HbA1c + 24h Urinary Free Cortisol

Frequently asked questions

What does an abnormal HbA1c or cortisol level mean?
An elevated HbA1c suggests poor blood sugar control, which may indicate diabetes or resistance to insulin. High cortisol in urine could signal chronic stress, adrenal issues, or conditions like Cushing’s syndrome. Together, these tests can help diagnose or monitor conditions affecting both blood sugar and stress hormones.
How often should I get this test?
HbA1c is typically checked every 3–6 months for people with diabetes. The 24-hour urine cortisol test is usually ordered if your doctor suspects hormonal imbalances or stress-related conditions. Follow your healthcare provider’s recommendations for timing and frequency.
Can diet or lifestyle changes affect these test results?
Yes. HbA1c can be influenced by diet, exercise, and medications like insulin or diabetes drugs. For cortisol, stress, caffeine, and certain medications (e.g., steroids) can alter urine levels. Your doctor may recommend adjustments to diet or timing of tests if needed.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy