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Cerebrospinal Fluid (CSF) Total Protein by Electrophoresis

Published: August 9, 2026Updated: August 16, 2026

This test measures the overall protein content in your cerebrospinal fluid (the fluid surrounding your brain and spinal cord) using electrophoresis, a method that separates proteins based on size and charge.

Quick summary

["Understanding your CSF protein levels can help diagnose conditions affecting your brain and nervous system."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
16594-4
Short code
C trach IgA Titr CSF IF
Synonyms
C trach IgA Titr CSF IF
Category
chemistry
Sample
csf
Container
Sterile tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
if
CPT
82784
Specimen volume
1-2 mL
Stability
Immediate analysis required, Refrigerated: 1h
Hemolysis max
200
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if you have symptoms like headaches, neurological issues, or signs of inflammation in the brain or spinal cord. It can also be part of investigations for conditions such as multiple sclerosis, infections, or tumors.

How the Test Works

A small amount of CSF is collected through a procedure called a lumbar puncture (spinal tap). The fluid is then analyzed using electrophoresis to separate proteins, which are measured for total protein content.

What to Expect Before the Test

You may be asked to fast before the test, but this is not always required. Wear comfortable clothing and inform your doctor if you have any concerns about the procedure or recent bleeding.

What to Expect During the Test

The procedure involves inserting a needle into your lower back to collect CSF. You may feel pressure or a slight sting, but it usually doesn’t cause significant discomfort. You’ll lie on your side with your knees bent.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

CSF Protein Profile

Frequently asked questions

How long does it take to get results?
Results are typically available within 24 to 48 hours, depending on lab processing times. Your doctor will discuss the findings with you during a follow-up visit.
What does it mean if my protein levels are high?
High protein levels in CSF can signal inflammation, infection, or other neurological disorders. Your doctor will review your symptoms and other test results to determine the next steps.
Do I need to prepare for this test in any special way?
No special preparation is usually needed, but avoid heavy lifting or strenuous activity for 24 hours before the test to reduce the risk of complications.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy