24-Hour Urinary Androstenedione

Why Is This Test Done?

Your healthcare provider may order a 24-hour urinary androstenedione test to check for hormonal imbalances. Common reasons include investigating symptoms like irregular periods, early puberty in children, or signs of adrenal or ovarian disorders. It can also help diagnose conditions like congenital adrenal hyperplasia (CAH) or other hormonal deficiencies.

How Is the Test Collected?

You’ll start collecting your urine in a special container at home, beginning right after waking up. You’ll keep the container with you and empty it into it every time you urinate throughout the day. Bring all collected urine to your lab in a sealed container by the next morning. Do not miss any urine—this ensures an accurate 24-hour measurement.

What Should You Expect During the Test?

There are no special preparations needed beyond collecting urine over 24 hours. You’ll need to follow a simple urine collection schedule, and your provider may ask you to avoid certain medications or foods that could interfere with results. Your sample will be analyzed in a lab to measure androstenedione levels.

What Do Results Mean?

Results show the total amount of androstenedione excreted in your urine over 24 hours. Higher levels may suggest overactivity of adrenal or ovarian glands, while lower levels could indicate hormonal deficiencies or other conditions. Your provider will interpret results in relation to your symptoms and medical history.