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Cerebrospinal Fluid (CSF) Protein Profile by Electrophoresis

Published: August 9, 2026Updated: August 16, 2026

This test measures the different types of proteins found in your cerebrospinal fluid (the fluid surrounding your brain and spinal cord) using gel electrophoresis. It helps identify abnormal protein patterns that may indicate conditions like multiple sclerosis, infections, or tumors.

Quick summary

["This test helps diagnose and monitor conditions affecting brain and spinal fluid chemistry."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
70929-5
Short code
Deprecated C-spine CT Stereo
Synonyms
Deprecated C-spine CT Stereo
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ct
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Does This Test Measure?

Electrophoresis separates proteins in your CSF based on size and electrical charge. The test detects distinct protein bands, including albumin, globulins, and other proteins, which can reveal imbalances linked to neurological or inflammatory diseases.

Why Might You Need This Test?

Your doctor may order this test if you have symptoms like headaches, vision changes, numbness, or neurological issues. It’s also used to monitor conditions like multiple sclerosis, meningitis, or brain tumors by examining protein patterns in CSF.

Common Conditions Monitored

Abnormal protein bands may signal conditions such as multiple sclerosis (where immunoglobulin G appears), infections (e.g., bacterial meningitis with high protein levels), or tumors (e.g., lymphoma with elevated protein fragments).

Sample Collection and Preparation

A lumbar puncture (spinal tap) is performed to collect CSF. The fluid is then analyzed in a lab using gel electrophoresis to separate proteins for evaluation.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

CSF Protein Electrophoresis

Frequently asked questions

How often do I need this test?
This test is typically done once or twice a year for chronic conditions like multiple sclerosis, but your doctor will determine frequency based on your specific diagnosis and symptoms. Follow-up may depend on treatment progress or new symptoms.
What are the risks of this test?
The most common risk is a mild headache or temporary back pain after the lumbar puncture. Rarely, complications like infection or nerve damage may occur, but serious risks are uncommon with proper medical supervision.
Can I eat or drink before this test?
You may eat and drink normally before the test unless instructed otherwise by your doctor. Avoid caffeine or alcohol for 24 hours before the procedure to minimize potential effects on CSF composition.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy