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Adrenal Cortisol Level (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cortisol, a hormone produced by your adrenal glands, in your blood. Cortisol helps regulate metabolism, immune response, and stress reactions.

Quick summary

["Cortisol helps manage stress and supports normal bodily functions; abnormal levels may signal health concerns."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
46367-9
Short code
Deprecated CT Adrenal Bx needle guide
Synonyms
Deprecated CT Adrenal Bx needle guide
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ct
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order a cortisol test to check for conditions like Cushing’s syndrome (too much cortisol), adrenal insufficiency (too little cortisol), or to monitor treatment for hormone-related disorders. It can also help diagnose issues with the pituitary gland or adrenal glands.

How the Test Is Taken

A blood sample is drawn from a vein, usually in your arm. You may feel slight discomfort, but it’s quick and typically done in a doctor’s office or lab.

What to Expect Before the Test

No special preparation is needed, but if you’re taking medications that affect cortisol levels (like steroids), your doctor may ask you to stop them temporarily or adjust timing.

What the Results Mean

Results are compared to standard ranges, but your doctor will interpret them based on your symptoms and medical history. Abnormal results may trigger further testing or follow-up.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Serum Cortisol

Frequently asked questions

How often should I get this test?
This test is usually done once or twice to confirm or rule out conditions. Your doctor will determine the frequency based on your health needs and symptoms.
Can I eat or drink before the test?
You can eat and drink normally unless instructed otherwise. Avoid caffeine or alcohol before the test, as they may affect cortisol levels temporarily.
What if my results are out of range?
If your cortisol levels are too high or too low, your doctor may recommend additional tests, like a 24-hour urine test or a dexamethasone suppression test, to get a clearer picture of your health.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy