Homocysteine (Total) with Methylcobalamin and Folate Status
This test measures your total homocysteine levels and evaluates whether your vitamin B12 (methylcobalamin) and folate (folic acid) levels are sufficient. Homocysteine is an amino acid that can build up in your blood if your body doesn’t have enough of these vitamins.
["Check for vitamin deficiencies and elevated homocysteine that may raise heart disease risk."]
Key facts at a glance
- LOINC
- 33061-3
- Short code
- Deprecated (Hcys)2 6h p Met-(Hcys)2 - CF
- Synonyms
- Deprecated (Hcys)2 6h p Met-(Hcys)2 - CF
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why This Test Is Done
Your doctor may order this test if you have symptoms of vitamin deficiencies (like fatigue, numbness, or memory issues) or if you have a history of heart disease, stroke, or other blood vessel problems. It can also be part of routine screening for people at higher risk of cardiovascular issues.
What Homocysteine Does in Your Body
Homocysteine is a natural substance your body makes from proteins. High levels can damage blood vessels over time, increasing the risk of heart disease, stroke, or blood clots. Your body needs enough B12 and folate to break down homocysteine properly.
How Vitamins B12 and Folate Play a Role
B12 (methylcobalamin) and folate help your body convert homocysteine back into healthy amino acids. If these vitamins are low, homocysteine stays in your blood longer, raising risks. This test checks both levels to see if they’re supporting your metabolism.
What to Expect Before the Test
You’ll likely have a simple blood draw from a vein in your arm. No special preparation is needed, but tell your doctor about any medications or supplements you take, especially those affecting blood clotting or homocysteine levels.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
◆Associated findings1
Short name
Total Homocysteine (with Vitamin B12/Folate Check)
Frequently asked questions
High homocysteine levels combined with low B12 or folate may signal an increased risk for cardiovascular diseases, nerve damage, or pregnancy complications. Early detection helps manage risk with diet, supplements, or lifestyle changes.
What does a high homocysteine level mean for my health?
Do I need to fast before this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy