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Maxillofacial Cortisol (24-Hour Urinary Free Cortisol)

Published: August 9, 2026Updated: August 17, 2026

This test measures the amount of cortisol, a natural steroid hormone, excreted in your urine over a 24-hour period. It helps assess your body’s cortisol production and can be used to diagnose conditions related to adrenal gland function or stress.

Quick summary

["This test helps evaluate adrenal function and stress responses."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
36957-9
Short code
Deprecated Maxillofacial CT +3DR
Synonyms
Deprecated Maxillofacial CT +3DR
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
80
Ref high
200
Critical range
20 N/A – 400 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ct
CPT
84436
SNOMED
302346007
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order a 24-hour urine cortisol test if they suspect conditions like Cushing’s syndrome (excess cortisol), adrenal insufficiency (too little cortisol), or other hormonal imbalances. It can also help diagnose issues related to chronic stress or pituitary disorders.

How the Test Works

You’ll collect all your urine for a full 24-hour period, typically starting and ending at the same time each day. The urine is stored in a special container, and the sample is sent to a lab for analysis to measure cortisol levels.

Sample Collection

To prepare, empty your bladder before starting the collection. Use a clean container and change it every time you urinate. Avoid drinking excessive fluids or taking certain medications that could affect cortisol levels. Your doctor will provide detailed instructions.

What to Expect After the Test

After collection, your urine sample is processed in a lab. Results are compared to standard ranges to determine if cortisol levels are within normal limits or if further evaluation is needed. Follow-up may include additional tests or consultations.

Reference range

Adult: 80-200 ng/dLN/A

Specimen: serum

Take your thyroid medication after the blood draw if applicable. No fasting required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24-Hour Urine Cortisol

Frequently asked questions

What does a high cortisol level mean?
High cortisol levels may indicate conditions such as Cushing’s syndrome, where the body produces too much cortisol, or chronic stress. This can lead to symptoms like weight gain, high blood pressure, or muscle weakness. Your doctor will discuss findings with you to determine the next steps.
What does a low cortisol level mean?
Low cortisol levels may suggest adrenal insufficiency, where the adrenal glands don’t produce enough cortisol. This can cause fatigue, dizziness, low blood pressure, or other symptoms. Treatment may involve hormone replacement therapy or further medical evaluation.
How often should I get this test?
The frequency depends on your symptoms and medical needs. Your doctor will decide based on your health history, current conditions, and any previous test results. Regular monitoring may be recommended for chronic stress or hormonal disorders.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy