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Total Protein with Electrophoresis Separation (Serum/Plasma)

Published: August 9, 2026Updated: August 16, 2026

This test measures the overall protein content in your blood and breaks down its components using electrophoresis to identify patterns that may help diagnose conditions affecting protein levels or distribution.

Quick summary

["Understanding your blood protein composition can help spot potential health issues early."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
99037-4
Short code
dfrA12 Islt/Spm Ql
Synonyms
dfrA12 Islt/Spm Ql
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
molgen
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Is Protein Electrophoresis Used For?

Electrophoresis helps separate blood proteins into fractions (like albumin, globulins, and other proteins) based on size and charge. This can reveal imbalances that may indicate conditions such as kidney disease, liver problems, infections, or certain cancers.

How Is This Test Performed?

A small blood sample is drawn, and the serum or plasma is analyzed using an electric field to separate proteins. The results show the distribution of different protein types, which can be compared to a reference pattern.

Why Might You Need This Test?

Your doctor may order this test if you have symptoms like swelling, unexplained weight loss, fatigue, or if they suspect conditions like multiple myeloma, chronic kidney disease, or autoimmune disorders that affect protein levels.

What to Expect Before the Test

There are no special preparations needed. You may have a slight bruise or soreness at the needle site, but it usually fades quickly. If you’re on blood thinners, let your doctor know before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Abnormal protein patterns can signal underlying health issues. For example, low albumin may indicate malnutrition or liver/kidney disease, while high globulin levels could suggest inflammation or immune disorders. This test helps guide further testing or treatment plans.

Short name

Total Protein Profile

Frequently asked questions

How long does it take to get results?
Results are typically available within 1–3 business days, depending on lab processing times. Your doctor will discuss them with you during your follow-up visit.
Can I eat or drink before this test?
No special diet is required. You can eat and drink normally before the blood draw, unless your doctor advises otherwise.
Is this test painful?
The blood draw itself may cause a brief sting, but most people tolerate it well. The discomfort is usually mild and temporary.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy