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24-Hour Total Dehydroepiandrosterone (DHEA) Urine Test

Published: August 9, 2026Updated: August 17, 2026

This test measures the total amount of dehydroepiandrosterone (DHEA), a hormone produced by your adrenal glands, over a 24-hour period in your urine.

Quick summary

["Assesses adrenal hormone function and may help identify conditions affecting hormone production."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
25895-4
Short code
DHEA 24h Ur-sCnc
Synonyms
DHEA 24h Ur-sCnc
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Ref low
135
Ref high
980
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82626
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What Is Dehydroepiandrosterone (DHEA)?

DHEA is a natural hormone made in your adrenal glands, which sit above your kidneys. It’s a precursor to other hormones, including testosterone and estrogen. Your body produces DHEA in large amounts, and small amounts are excreted in urine over 24 hours. This test helps doctors evaluate adrenal health and hormone balance.

Why Might You Need This Test?

Your doctor may order a 24-hour DHEA urine test if they suspect conditions like adrenal insufficiency, Cushing’s syndrome, or other hormonal imbalances. It can also be part of a broader evaluation for chronic fatigue, autoimmune disorders, or unexplained weight changes.

How Is the Test Performed?

You’ll collect all your urine over a full 24-hour period, typically starting after midnight the night before the test. Empty your bladder at the start, then collect every urine sample in a container until you’ve passed urine again the next morning. The collected urine is then sent to a lab for analysis.

What to Expect During Collection

To ensure accuracy, follow your doctor’s instructions carefully. Wear comfortable clothing and plan for the day to include bathroom breaks. If you’re pregnant, breastfeeding, or have certain medications, let your doctor know—these can affect results.

Reference range

NCBI: DHEA-S Male 135-980 µg/dL; Female 30-260 µg/dLN/A

Specimen: urine

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

DHEA 24h Urine

Frequently asked questions

What Does a Low or High Result Mean?
Low levels of DHEA in urine may indicate adrenal gland problems, such as insufficient production of hormones (adrenal insufficiency) or conditions like Addison’s disease. High levels could suggest overactivity of the adrenal glands or other metabolic issues, though this is less common.
How Does This Test Compare to Other Tests?
Unlike blood tests for DHEA, this urine test provides a more comprehensive picture of your body’s daily hormone excretion. Blood tests measure DHEA at a single point in time, while urine tests reflect long-term hormone balance.
Can I Take Medications or Supplements Before the Test?
Yes, but inform your doctor about any medications, supplements, or over-the-counter drugs you’re taking. Some medications, like steroids or certain blood pressure drugs, can affect DHEA levels and may require adjustments or timing changes for your test.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy