Skip to content

24-Hour Urinary Free Cortisol (Total)

Published: August 9, 2026Updated: August 17, 2026

This test measures the total amount of cortisol excreted in your urine over a 24-hour period. Cortisol is a hormone produced by your adrenal glands that helps regulate stress responses and metabolism.

Quick summary

["Helps assess adrenal gland function and cortisol metabolism over time."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
57786-6
Short code
DHEA 2D p high Dex 24h Ur-mRate
Synonyms
DHEA 2D p high Dex 24h Ur-mRate
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Ref low
135
Ref high
980
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82626
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if they suspect conditions like Cushing’s syndrome (too much cortisol), adrenal insufficiency (too little cortisol), or other hormonal imbalances. It can also be used to monitor treatment effectiveness for conditions affecting cortisol levels.

What to Expect Before the Test

You’ll need to collect all urine for a full 24-hour period, typically starting after midnight on one day and ending midnight the next. Follow your healthcare provider’s instructions carefully to ensure accurate results. Drink plenty of fluids to help urine flow, but avoid caffeine or alcohol, as they can affect results.

How the Test Is Collected

Urine is collected in a special container. You’ll empty your bladder at the start of the collection period, then collect every urine void into the container for the full 24 hours. Some labs may provide a timed collection kit to simplify the process.

What the Results Mean

Results are compared to standard ranges for healthy adults. High levels may indicate overproduction of cortisol, while low levels may suggest adrenal gland dysfunction. Your doctor will interpret results in the context of your symptoms and medical history.

Reference range

NCBI: DHEA-S Male 135-980 µg/dL; Female 30-260 µg/dLN/A

Specimen: urine

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24h Free Cortisol Urine

Frequently asked questions

What questions might you have about this test?
This test is primarily used to diagnose or monitor conditions related to cortisol production. It helps rule out conditions like Cushing’s syndrome, adrenal insufficiency, or congenital adrenal hyperplasia. If you have symptoms like unexplained weight gain, fatigue, or mood changes, your doctor may recommend this test.
How long will I need to prepare for this test?
Preparation depends on your specific instructions, but generally, you’ll need to collect urine for 24 hours straight. Your lab technician or doctor will provide detailed guidance on timing, diet, and other precautions to follow.
Can I take medications or supplements before this test?
Some medications or supplements, like certain steroids or caffeine, can affect cortisol levels. Always check with your doctor or lab technician before the test to confirm any restrictions on your usual medications or supplements.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy