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Total Protein with Albumin/Globulin Separation (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures the total protein in your blood and breaks it down into albumin and globulin fractions, which help assess protein balance and potential liver or kidney issues.

Quick summary

["Understanding your blood’s protein makeup can help detect health problems early."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
104683-8
Short code
DMA 18:0 DBS-mCnc
Synonyms
DMA 18:0 DBS-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Matters

Total protein levels reflect how well your body is retaining and distributing essential nutrients. Albumin, the main protein in blood, helps maintain fluid balance and supports tissue repair. Globulins, which include antibodies and transport proteins, play roles in immunity and hormone regulation. Abnormal results may signal conditions like malnutrition, liver disease, or kidney problems.

How the Test Works

A small blood sample is drawn and analyzed using a specialized technique to separate and measure protein components. This helps identify imbalances that might not be detected by a simple total protein test alone.

What to Expect Before the Test

No special preparation is needed, but you may want to avoid heavy meals 2 hours before your appointment. Wear comfortable clothing if you’ll need an arm restraint for the blood draw.

Common Results and Next Steps

If your results show unusually high or low protein levels, your healthcare provider may recommend further testing to investigate potential causes, such as chronic diseases or dietary concerns.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: Protein Profile Results Can Indicate Underlying Conditions

Short name

Protein Profile

Frequently asked questions

What causes abnormal protein levels?
Abnormal protein results may be caused by conditions like liver disease (affecting albumin production), kidney disease (leading to protein loss in urine), malnutrition, or certain autoimmune disorders. High globulin levels could signal chronic infections or inflammation, while low albumin might indicate liver or kidney dysfunction.
Can diet affect protein test results?
Dietary changes can influence protein levels, especially if you have conditions like liver disease or kidney problems. Your provider may recommend dietary adjustments or monitoring if protein imbalances are detected.
How often should I get this test?
Your provider will determine how often to repeat this test based on your health status, symptoms, or known conditions. Regular monitoring may be needed if you have chronic illnesses like liver disease or kidney disease.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy