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DNA Index (Cell Cycle Analysis) via Flow Cytometry

Published: August 9, 2026Updated: August 16, 2026

This test measures the DNA content of cells to assess their stage in the cell cycle, often used to evaluate abnormal cell proliferation.

Quick summary

["Helps doctors understand if cells are growing in a healthy or potentially cancerous way."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
60253-2
Short code
DNA index 4 Spec FC-aCnc
Synonyms
DNA index 4 Spec FC-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
flow-cytometry
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Is DNA Index Testing Used For?

DNA index testing is primarily used in **oncology** to determine whether cells are dividing abnormally, which can be a sign of cancer. It’s often performed on tissue samples (e.g., from a biopsy) to guide treatment decisions, such as chemotherapy or surgery.

How Is This Test Performed?

A small sample of cells (e.g., from a tumor or other tissue) is collected during a biopsy or surgical procedure. The cells are then analyzed using **flow cytometry**, a technique that measures DNA content to classify them by their cell cycle phase.

Sample Collection and Preparation

No special preparation is needed before the biopsy. The test itself requires a fresh tissue sample, which is processed immediately to preserve cell integrity. Results are typically reported as a numerical index (e.g., 1.0 for diploid cells, >1.0 for aneuploid cells).

What Do Results Mean?

Results are interpreted in the context of the clinical situation. A **DNA index >1.0** often suggests cells are dividing uncontrollably, which may indicate malignancy. A normal index (e.g., 1.0) suggests cells are growing in a more typical, non-cancerous pattern.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

DNA Index

Frequently asked questions

Why Might I Need This Test?
Your doctor may recommend this test if you have a suspected tumor, abnormal growth, or symptoms that suggest an underlying cancer. It helps assess whether cancer cells are present and may guide decisions about further testing or treatment options.
What Are the Possible Risks or Side Effects?
The biopsy procedure carries minor risks like pain, bruising, or infection at the site. The DNA index test itself has no direct risks, but results may influence treatment decisions that could include surgery, radiation, or chemotherapy.
How Long Does It Take to Get Results?
Results are usually available within **1–2 weeks**, depending on lab processing times and clinical workflows. Follow-up with your healthcare provider will help interpret the findings in relation to your diagnosis.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy