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Escherichia coli in Stool (Qualitative, Probe-Based)

Published: August 9, 2026Updated: August 16, 2026

This test checks for the presence of *Escherichia coli* bacteria in your stool, which can help identify infections caused by this common gut bacterium.

Quick summary

["A positive result may indicate a bacterial infection needing medical attention."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
61398-4
Short code
E coli DNA Spec Ql NAA+probe
Synonyms
E coli DNA Spec Ql NAA+probe
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe-amp-tar
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms like diarrhea (especially with blood or mucus), abdominal pain, fever, or if you’ve recently traveled to an area with higher risk of food/waterborne illnesses. It’s also used to diagnose or monitor conditions like traveler’s diarrhea, urinary tract infections (UTIs), or other gastrointestinal infections.

How the Test Works

A small sample of your stool is collected and tested using a specialized probe method to detect *E. coli* DNA. This is a qualitative test, meaning it only tells you whether the bacteria are present or not, not how many are present.

Sample Collection

You’ll need to provide a fresh stool sample (usually collected in a sterile container) during your visit. Follow your provider’s instructions carefully to ensure the sample is representative.

Test Results Interpretation

A positive result means *E. coli* was found in your stool. This could indicate an infection, but other causes (like food poisoning or certain medications) may also be possible. A negative result means no *E. coli* was detected, but other bacteria or viruses could still be causing symptoms.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

E. coli Stool Culture

Frequently asked questions

What Does a Positive Result Mean?
A positive result suggests you may have an *E. coli* infection, which can range from mild stomach upset to severe illnesses (like EHEC, which can cause life-threatening complications). It’s important to see a doctor to determine the best treatment, which may include antibiotics, hydration support, or other interventions depending on severity.
What Does a Negative Result Mean?
A negative result means *E. coli* wasn’t detected in this sample, but other pathogens (like Salmonella, Shigella, or viruses) could still be causing your symptoms. If symptoms persist, further testing may be needed.
When Might You Need Follow-Up Testing?
If you have ongoing symptoms after a negative result, your provider may recommend additional tests (like bloodwork, imaging, or repeat stool cultures) to identify the cause of your illness. If you’re prescribed antibiotics, follow your doctor’s instructions carefully to avoid antibiotic-resistant bacteria.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy