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Escherichia coli (E. coli) bacterial culture and antibiotic susceptibility screening (serum/plasma)

Published: August 9, 2026Updated: August 16, 2026

This test checks for the presence of *Escherichia coli* bacteria in your blood, which can indicate serious infections like sepsis. It also helps determine if antibiotics are effective against these bacteria.

Quick summary

["A positive result may signal a blood infection requiring urgent medical attention and tailored antibiotic treatment."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
44087-5
Short code
E coli O157 Ag Spec Ql
Synonyms
E coli O157 Ag Spec Ql
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The test involves culturing blood samples to identify *E. coli* bacteria. If bacteria are found, further testing (like antibiotic susceptibility) helps guide treatment choices. This is typically done using a blood culture method, which may take several days to complete.

Why It’s Done

Doctors order this test if you have symptoms like fever, chills, rapid heartbeat, or confusion—common signs of a blood infection. It’s also used when you’re at higher risk, such as after surgery, chemotherapy, or if you have weakened immune systems.

Sample Collection and Process

A blood sample is drawn from a vein, usually in your arm. The sample is then sent to a lab where it’s cultured in special growth media to detect *E. coli* and other bacteria. If bacteria are present, they’ll grow into colonies that can be identified under a microscope or tested further.

Antibiotic Sensitivity Testing

If *E. coli* is confirmed, additional testing may be done to see which antibiotics work best against it. This involves exposing the bacteria to different antibiotics and measuring how they grow or survive. This helps prevent antibiotic resistance.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

E. coli in Blood

Frequently asked questions

Early detection of *E. coli* in the blood is critical because untreated infections can lead to severe complications, including organ failure or death. This test helps doctors diagnose sepsis quickly and start appropriate treatment, such as intravenous antibiotics.
A positive result means you likely have a blood infection caused by *E. coli*. Your doctor will work with you to choose the best antibiotics based on the test results and your overall health.
How long will I need to wait for results?
Blood culture results can take 2–5 days to confirm bacterial presence, while antibiotic susceptibility testing may take an additional 2–3 days. Your doctor will discuss timelines with you during your visit.
What are the risks or side effects of this test?
The main risk is a mild bruise or soreness at the injection site. Rarely, there may be a reaction to the blood draw, such as fainting or swelling. If you have a bleeding disorder or take blood thinners, let your doctor know before the test.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy