Antibody Detection by Line Immunoblot for Autoantibodies (e.g., Rheumatoid Factor, Antinuclear Antibodies)
This test measures specific antibodies in your blood that may indicate autoimmune conditions, such as lupus or rheumatoid arthritis, by detecting proteins that react with certain antigens on a test strip.
["Helps identify potential autoimmune activity in your body."]
Key facts at a glance
- LOINC
- 89529-2
- Short code
- ENA Jo1 Ab CSF Ql Line blot
- Synonyms
- ENA Jo1 Ab CSF Ql Line blot
- Category
- chemistry
- Sample
- csf
- Container
- Sterile tube
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- line-blot
- Specimen volume
- 1-2 mL
- Stability
- Immediate analysis required, Refrigerated: 1h
- Hemolysis max
- 200
- TAT
- 4–24 hours
- Order
- routine
What This Test Measures
The test uses a line blot method to detect a range of autoantibodies, including those commonly associated with autoimmune diseases. It identifies specific bands (lines) where antibodies bind to antigens, helping to confirm or rule out conditions like systemic lupus erythematosus (SLE) or other autoimmune disorders.
Why It Might Be Ordered
Your healthcare provider may recommend this test if you have symptoms like joint pain, fatigue, rash, or unexplained inflammation, or if you have a family history of autoimmune diseases. It’s also used to monitor disease activity in patients already diagnosed with autoimmune conditions.
How the Test Works
A small blood sample is drawn and analyzed in a lab. The sample is applied to a specialized blot containing multiple antigens. Antibodies in your blood bind to these antigens, creating visible lines that indicate the presence of specific autoantibodies. This method is highly sensitive and can detect low levels of antibodies.
Preparation and Sample Collection
No special preparation is needed for this test. You may have a simple venipuncture (blood draw) at a clinic or lab. If you’re on blood thinners, inform your provider beforehand.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Positive results for specific autoantibodies can support a diagnosis of autoimmune diseases, guide treatment decisions, or help track disease progression. Negative results may rule out certain autoimmune conditions, but they don’t rule out all possibilities. Always discuss results with your healthcare provider for personalized interpretation.
Short name
Autoantibody Profile (Line Blot)
Frequently asked questions
What does a positive result mean?
Can this test detect all autoimmune diseases?
How long does it take to get results?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy