Skip to content

Antibody Screening and Confirmation for Autoimmune or Infectious Conditions via Line Blot Immunoassay

Published: August 9, 2026Updated: August 16, 2026

This test detects specific antibodies in your blood that may indicate an autoimmune disease or an infection. It uses a line blot method to identify certain proteins or microbes.

Quick summary

["This test helps doctors diagnose conditions like lupus, rheumatoid arthritis, or infections by checking for unusual antibody responses."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
99069-7
Short code
ENA Jo1 Ab Ser Line blot-aCnc
Synonyms
ENA Jo1 Ab Ser Line blot-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
line-blot
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The test detects antibodies bound to specific targets on a membrane, creating visible lines. These lines indicate the presence of particular proteins or microbial components in your bloodstream. This method is sensitive and can identify multiple antibodies at once.

How the Test Works

A small blood sample is taken, and antibodies in your serum or plasma react with predefined antigens on a test strip. If antibodies are present, they bind to these antigens, forming visible lines that are detected and analyzed.

Why It’s Done

Doctors may order this test if you have symptoms like joint pain, fatigue, or unexplained infections, or if they suspect an autoimmune disease or chronic infection.

Common Conditions It Helps Diagnose

This test can help identify conditions such as autoimmune diseases (e.g., lupus, rheumatoid arthritis), infections (e.g., Lyme disease, hepatitis), or other immune-related disorders by detecting abnormal antibody responses.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Autoimmune/Infectious Antibody Panel

Frequently asked questions

Why might I need this test?
Your healthcare provider may recommend this test if you have symptoms like persistent fatigue, unexplained joint pain, or a history of recurrent infections. It helps rule out autoimmune or infectious causes of these symptoms.
What does a positive result mean?
A positive result indicates the presence of antibodies against specific targets, which may suggest an autoimmune condition or infection. However, results alone do not diagnose a condition; they guide further testing and evaluation by your doctor.
How long does it take to get results?
Results are typically available within 24 to 48 hours, depending on lab processing times. Your doctor will discuss the findings with you during your follow-up appointment.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy