Enzyme-linked immunosorbent assay (ELISA) for anti-neutrophil cytoplasmic antibody (ANCA) subtypes (p-ANCA and c-ANCA)
This test measures specific antibodies in your blood that target proteins in neutrophils, a type of white blood cell. These antibodies can indicate certain autoimmune conditions, such as vasculitis.
["Helps diagnose and monitor autoimmune diseases linked to ANCA-positive vasculitis."]
Key facts at a glance
- LOINC
- 53008-9
- Short code
- ENA PM/SCL Ab Titr Ser IF
- Synonyms
- ENA PM/SCL Ab Titr Ser IF
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- if
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Your healthcare provider ordered this test to help check how your body is functioning and to look for signs of a specific condition. The results give important information that supports an accurate diagnosis, guides treatment decisions, and helps your care team monitor your overall health over time.
Your doctor may order this test if you have symptoms like joint pain, skin rashes, kidney problems, or unexplained infections. It’s also used to check if your condition is active or improving after treatment.
This test works by measuring specific substances or markers in your body and comparing the values with established reference ranges. A laboratory analyzes your sample using specialized equipment, and the results help your healthcare provider determine whether everything appears within a normal range or whether further evaluation is needed.
A small blood sample is taken and tested for two main types of ANCA antibodies: p-ANCA (proteinase 3) and c-ANCA (myeloperoxidase). The ELISA method detects these antibodies by measuring their presence and concentration.
A trained professional will collect your sample, usually at a laboratory, clinic, or hospital, using standard procedures designed to keep you comfortable and safe. Before your appointment, follow any instructions from your healthcare provider, such as fasting for a set number of hours, avoiding certain foods or medications, or drinking plenty of water, because good preparation helps ensure accurate results.
Blood is drawn from a vein, usually in your arm, and sent to a lab. The serum (liquid part of blood without clots) is used for testing.
Before the test, your healthcare provider will review your medical history, current medications, and any symptoms you may have, and will explain the purpose of the test and answer your questions. You may be asked to sign a consent form and share details about allergies or past reactions to blood draws. Plan to arrive a little early, wear comfortable clothing, and let the staff know if you feel anxious so they can help you stay relaxed.
No special preparation is needed, but inform your doctor about any medications, especially blood thinners, that you’re taking.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical Significance
Short name
ANCA Antibody Screening/Subtype Confirmation
Frequently asked questions
ANCA antibodies are linked to a group of inflammatory diseases called vasculitis, which cause inflammation in blood vessels. Detecting these antibodies helps confirm a diagnosis and guides treatment decisions.
A positive result suggests you may have an ANCA-associated vasculitis, but further testing and clinical evaluation are needed to confirm the diagnosis and determine the best treatment plan.
Yes, ANCA levels can be monitored over time to track disease activity and response to treatment, though results should always be interpreted by your healthcare provider.
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy