Protein Electrophoresis (Serum/Plasma) with Immunofixation (IEF)
This test measures the distribution of proteins in your blood, including identifying specific proteins that may be abnormal, such as monoclonal proteins (often seen in conditions like multiple myeloma).
["Helps diagnose and monitor blood disorders by detecting unusual protein patterns or specific antibodies."]
Key facts at a glance
- LOINC
- 89535-9
- Short code
- ENA RNP Ab CSF Ql Line blot
- Synonyms
- ENA RNP Ab CSF Ql Line blot
- Category
- chemistry
- Sample
- csf
- Container
- Sterile tube
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- line-blot
- Specimen volume
- 1-2 mL
- Stability
- Immediate analysis required, Refrigerated: 1h
- Hemolysis max
- 200
- TAT
- 4–24 hours
- Order
- routine
What Does This Test Measure?
The test separates blood proteins into different fractions (e.g., albumin, globulins) using electrophoresis. Immunofixation identifies any abnormal proteins that may appear as bands or spikes on the gel, which could indicate conditions like monoclonal gammopathy or other immune system issues.
Why Might You Need This Test?
Your doctor may order this test if you have symptoms like unexplained fatigue, frequent infections, kidney problems, or if they suspect a condition like multiple myeloma, lymphoma, or Waldenström macroglobulinemia. It’s also used to monitor treatment progress.
Sample Collection and Preparation
A blood sample is drawn from a vein, usually in your arm. The blood is processed to separate serum or plasma, which is then analyzed on an electrophoresis gel. Immunofixation involves applying specific antibodies to detect any abnormal proteins.
Common Conditions Monitored
This test helps diagnose or evaluate conditions such as multiple myeloma (a cancer of plasma cells), monoclonal gammopathy of undetermined significance (MGUS), Waldenström macroglobulinemia, and other plasma cell disorders. It can also identify autoimmune diseases or chronic infections.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
◆Associated findings1
Short name
Protein Electrophoresis with Immunofixation
Frequently asked questions
How long will it take to get my results?
Abnormal results could indicate conditions like multiple myeloma, lymphoma, or other plasma cell disorders. They may also suggest chronic infections, autoimmune diseases, or other metabolic issues. Your doctor will interpret the results in the context of your symptoms and medical history.
No special preparation is usually needed for this test. You can eat and drink normally, but avoid smoking or consuming alcohol for at least 24 hours before your blood draw if instructed by your doctor.
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy