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Serum or plasma measurement of substance concentration for anti-nuclear antibodies (ANA) by immunoassay

Published: August 9, 2026Updated: August 16, 2026

This test checks for antibodies that attack healthy cells in your body, often linked to autoimmune conditions.

Quick summary

["Helps identify possible autoimmune diseases like lupus or rheumatoid arthritis."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
5301-7
Short code
ENA RNP Ab Ser Ql IA
Synonyms
ENA RNP Ab Ser Ql IA
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ia
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Does This Test Measure?

The test detects the presence of anti-nuclear antibodies (ANA), which target parts of your cells’ nucleus. These antibodies can be found in higher amounts in people with certain autoimmune diseases, where the immune system mistakenly attacks healthy tissues.

Why Might You Need This Test?

Your doctor may order this test if you have symptoms like joint pain, fatigue, rash, or other signs of autoimmune conditions. It can also be used as part of a screening or monitoring plan for conditions like lupus, scleroderma, or mixed connective tissue disease.

What to Expect Before the Test

No special preparation is usually needed. You may fast for 8–12 hours before the blood draw if instructed, but always follow your doctor’s instructions. Bring any medications you’re taking, as some may affect results.

What Happens During the Test?

A healthcare provider will take a blood sample from a vein in your arm. You may feel a slight pinch, but it’s quick and painless. The sample is then sent to a lab for analysis using an immunoassay method to detect ANA.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

ANA (Serum/Plasma)

Frequently asked questions

What Are Common Symptoms That Might Lead to This Test?
Symptoms like joint pain, unexplained fatigue, skin rashes, fever, or swelling in hands/feet could trigger this test. Your doctor may also order it if you have a family history of autoimmune diseases or if you’re being evaluated for unexplained symptoms.
How Are Results Interpreted?
Results are reported as a positive or negative for ANA presence. A positive result means antibodies were detected, but further testing (like specific antibody subtypes) is often needed to determine the exact cause. Your doctor will discuss results in the context of your symptoms and medical history.
Can This Test Be Used to Diagnose Autoimmune Diseases?
No, this test alone isn’t diagnostic. It’s often part of a broader evaluation that includes clinical symptoms, physical exams, and other tests (like rheumatoid factor or specific antibody panels) to narrow down possible conditions.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy