Skip to content

Serum or Plasma Protein Electrophoresis (Qualitative) with Bromocresol Green Staining

Published: August 9, 2026Updated: August 16, 2026

This test measures the distribution of different protein types in your blood, often used to check for conditions affecting protein levels or patterns, such as multiple myeloma or other blood disorders.

Quick summary

["Helps identify unusual protein patterns that may signal underlying health concerns."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
63542-5
Short code
ENA Scl70 Ab Fld Ql
Synonyms
ENA Scl70 Ab Fld Ql
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The test uses a technique called electrophoresis to separate proteins in your blood based on size and charge. Bromocresol Green dye binds to specific proteins, allowing for visual differentiation of protein bands. This helps detect abnormalities in protein concentration or distribution that may indicate conditions like monoclonal gammopathy or protein-losing syndromes.

Why It’s Ordered

Your healthcare provider may order this test if you have symptoms like unexplained weight loss, fatigue, frequent infections, or swelling. It’s also used as part of screening or monitoring for conditions like multiple myeloma, amyloidosis, or certain types of lymphoma.

Sample Collection and Preparation

A blood sample is drawn from a vein, typically in your arm. The blood is then processed to separate serum or plasma, which is sent to the lab for analysis. The sample is mixed with Bromocresol Green dye and placed in an electric field to separate proteins.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to avoid certain medications or foods that could interfere with results. If you’re on blood thinners, let your provider know before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Abnormal protein patterns detected in this test can signal conditions such as monoclonal gammopathy (where abnormal proteins accumulate), protein-losing disorders (like nephrotic syndrome), or systemic diseases like multiple myeloma or amyloidosis. Early detection can guide treatment decisions and monitor disease progression.

Short name

Bromocresol Green Protein Electrophoresis

Frequently asked questions

How long does it take to get results from this test?
Results are typically available within 1 to 3 business days, depending on lab processing times. Your healthcare provider will discuss the findings with you during your follow-up visit.
Can I eat or drink anything before this test?
No special diet is required, but avoid alcohol or certain medications that might affect protein levels. If you’re unsure, ask your provider for specific instructions.
What does it mean if my protein electrophoresis shows unusual bands?
Unusual bands may indicate an abnormal protein production, such as monoclonal spikes (common in multiple myeloma) or altered protein distribution. Your provider will interpret the results in the context of your symptoms and medical history to determine next steps.
  1. 1
  2. 2
  3. 3

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy