Antibody Detection by Line Blot Assay (e.g., for Anticardiolipin or Lupus Anticoagulant Screening)
This test detects specific antibodies in your blood using a line blot method. It may be used to screen for autoimmune conditions or other immune-related disorders.
["This test helps identify certain antibodies that could signal underlying health conditions."]
Key facts at a glance
- LOINC
- 89502-9
- Short code
- ENA Scl70 Ab Ser Line blot-aCnc
- Synonyms
- ENA Scl70 Ab Ser Line blot-aCnc
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- line-blot
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
What This Test Measures
The line blot assay identifies antibodies bound to specific antigens on a membrane. Each line represents a different target protein or antigen, allowing for qualitative detection of immune responses. Results are visually interpreted as the presence or absence of lines corresponding to specific antibodies.
Why It’s Ordered
Your healthcare provider may order this test if you have symptoms of autoimmune diseases (e.g., lupus, rheumatoid arthritis), recurrent blood clots, or other suspected immune system dysfunctions. It may also be part of a broader diagnostic workup for unexplained conditions.
Sample Collection and Preparation
Blood is drawn from a vein, typically in a clinic or lab. The sample is processed and tested for antibodies against specific antigens. Results are qualitative (positive/negative) rather than quantitative.
Interpretation and Next Steps
A positive result indicates the presence of certain antibodies, which may warrant further testing (e.g., confirmatory assays or additional bloodwork) to determine the underlying cause. A negative result suggests no detectable antibodies for those targets at this time.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical Significance: This test is primarily used as a screening tool. Positive findings may prompt further evaluation to diagnose conditions like lupus anticoagulant syndrome, anticardiolipin antibodies, or other autoimmune disorders. Always discuss results with your healthcare provider, as interpretation depends on clinical context and symptoms.
Short name
Line Blot Antibody Test
Frequently asked questions
What does a positive result mean?
How long does it take to get results?
Can I fast before this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy