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Serum or Plasma Substance Concentration (Bromocresol Green Dye Binding)

Published: August 9, 2026Updated: August 16, 2026

This test measures how bromocresol green dye binds to proteins in your blood, often used to assess liver function or protein synthesis.

Quick summary

["This test helps doctors check liver health or protein levels in your blood."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
53017-0
Short code
ENA SS-A 52kD Ab Ser-aCnc
Synonyms
ENA SS-A 52kD Ab Ser-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The bromocresol green (BCG) dye test evaluates protein binding capacity in your serum or plasma. When BCG dye is added, healthy proteins (like albumin) bind to it, changing its color. The test measures how much dye remains unbound, reflecting overall protein availability or liver function.

Why It’s Done

Doctors may order this test to diagnose or monitor conditions affecting protein production (e.g., liver disease, malnutrition) or to assess albumin levels, which support fluid balance and nutrient transport in your body.

Sample Collection and Preparation

A blood sample is drawn from a vein, typically in the arm. The sample is processed to separate serum (liquid after clotting) or plasma (liquid after anticoagulant use) for testing.

Common Conditions Monitored

This test is less commonly used today but historically helped identify liver dysfunction or hypoalbuminemia (low albumin). Modern tests like albumin levels or liver enzymes are preferred for most clinical needs.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

BCG Dye Binding Test

Frequently asked questions

Why might my doctor request this test?
Your doctor may request this test if they suspect liver disease, protein deficiency, or other conditions affecting protein metabolism—though newer tests are often preferred for clarity.
How long will it take to get results?
Results are typically available within 24 to 48 hours, depending on lab processing times.
What does a low or high result mean?
A low result may indicate liver disease, malnutrition, or protein loss, while a high result could suggest normal protein levels or specific metabolic conditions. Always discuss results with your healthcare provider.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy