Antibody Detection by Line Blot for Anticardiolipin (IgG and IgM) Antibodies

What This Test Measures

The test detects anticardiolipin antibodies—proteins your immune system produces against a specific phospholipid found in blood cells. These antibodies can be part of an autoimmune response or linked to conditions like antiphospholipid syndrome (APS). The test uses a line blot method to identify IgG and IgM antibody types.

Why It’s Ordered

Your doctor may recommend this test if you have symptoms like unexplained blood clots, recurrent miscarriages, or signs of lupus (e.g., joint pain, fatigue). It’s also used to monitor or confirm suspected antiphospholipid syndrome or other autoimmune conditions.

Sample Collection and Process

A blood sample is drawn to collect serum or plasma. The sample is then analyzed using a line blot immunoassay to detect specific IgG and IgM antibodies against cardiolipin. Results are reported as qualitative (positive/negative) or, in some cases, semi-quantitative.

Interpreting Your Results

Results are typically qualitative, meaning they indicate whether antibodies are present (positive) or not (negative). A positive result suggests a higher likelihood of an autoimmune or clotting-related condition, but further testing may be needed for diagnosis.