Serum Antibody Detection by Line Blot (for Anticardiolipin Antibodies)
This test checks for antibodies against cardiolipin, a fatty substance found in cell membranes, which may be linked to certain autoimmune conditions.
["Helps identify potential autoimmune or blood-clotting disorders."]
Key facts at a glance
- LOINC
- 89527-6
- Short code
- ENA SS-A Ab CSF Ql Line blot
- Synonyms
- ENA SS-A Ab CSF Ql Line blot
- Category
- chemistry
- Sample
- csf
- Container
- Sterile tube
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- line-blot
- Specimen volume
- 1-2 mL
- Stability
- Immediate analysis required, Refrigerated: 1h
- Hemolysis max
- 200
- TAT
- 4–24 hours
- Order
- routine
What This Test Measures
The test detects the presence of anticardiolipin antibodies in your serum. These antibodies are proteins your immune system produces in response to cardiolipin, a component found in cell membranes. The line blot method visualizes specific antibody binding to cardiolipin antigens on a test strip, allowing for a qualitative assessment of antibody levels.
Why It Might Be Ordered
Your healthcare provider may recommend this test if you have symptoms or risk factors for autoimmune diseases, such as lupus, antiphospholipid syndrome, or other conditions associated with abnormal blood clotting. It may also be used as part of a broader evaluation for unexplained clotting events or recurrent miscarriages.
Sample Collection and Preparation
A blood sample is drawn from a vein, typically in your arm. The blood is processed to separate serum, which is then tested for anticardiolipin antibodies. This test does not require fasting or special preparation beyond standard blood draw procedures.
Interpretation and Results
Results are reported qualitatively (positive or negative) based on whether antibodies are detected. A positive result indicates the presence of anticardiolipin antibodies, which may warrant further testing or evaluation by your healthcare provider.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Anticardiolipin antibodies are often associated with autoimmune conditions that can affect blood clotting. While a positive result does not definitively diagnose a specific condition, it may prompt additional testing to assess for conditions like antiphospholipid syndrome, lupus, or other autoimmune disorders. Your healthcare provider will interpret results in the context of your symptoms and medical history.
Short name
Anticardiolipin Antibody Screening (qualitative)
Frequently asked questions
What does a positive result mean?
Can this test be used to diagnose lupus?
Is fasting required for this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
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Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy