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Serum or Plasma Substance Concentration (Bromocresol Green Dye Binding)

Published: August 9, 2026Updated: August 16, 2026

This test measures how much of a specific protein in your blood binds to bromocresol green dye. It helps assess protein levels, particularly albumin, which is important for maintaining fluid balance and protecting tissues.

Quick summary

["This test can help diagnose or monitor conditions affecting protein levels in your blood."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
63346-1
Short code
ENA SS-A Ab Fld Ql ID
Synonyms
ENA SS-A Ab Fld Ql ID
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
immune-diffusion
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The bromocresol green (BCG) dye binding test evaluates the concentration of proteins in your blood, particularly albumin. When BCG dye binds to albumin, it changes color. The test measures how much dye binds to albumin, indirectly reflecting your albumin levels. This method is often used to check for conditions that alter protein synthesis or loss in the body.

Why It’s Done

Doctors may order this test to diagnose or monitor conditions like malnutrition, liver disease, kidney disease, or severe infections. It can also help assess how well your body is retaining fluids or protecting tissues from damage.

Common Uses and Conditions

This test is sometimes used in research or specialized clinical settings to evaluate protein status, though it is less common than direct albumin measurements. It may be part of broader testing for conditions affecting protein metabolism.

Sample Collection and Preparation

A blood sample is drawn from a vein, typically in the arm. The sample is then processed to separate serum or plasma, which is tested for its ability to bind bromocresol green dye. The test does not require fasting unless specified by your healthcare provider.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

BCG Dye Binding Test

Frequently asked questions

What does a low or high result mean?
A low result may suggest conditions like liver disease, kidney disease, or malnutrition, where the body cannot produce enough protein. A high result is less commonly significant but could indicate dehydration or specific genetic conditions. Always discuss results with your healthcare provider.
How often is this test performed?
This test is not routinely performed in most clinical settings due to its limited use compared to direct albumin measurements. It may be used in specialized research or specific clinical situations where protein binding assays are preferred.
Can I eat or drink before this test?
Typically, no special preparation is needed, but follow any instructions your healthcare provider gives you. If fasting is required, they will let you know.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy