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Serum or Plasma Substance Concentration (Bromocresol Green Dye Binding)

Published: August 9, 2026Updated: August 16, 2026

This test measures how bromocresol green (BCG) dye binds to proteins in your blood, often used to assess protein levels or liver function.

Quick summary

["This test helps doctors check how well your liver or protein production is working."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
33613-1
Short code
ENA SS-B IgG Ser-aCnc
Synonyms
ENA SS-B IgG Ser-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82784
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

Bromocresol green (BCG) is a dye that binds to proteins in your blood, including albumin. When your blood is mixed with BCG, the dye binds to proteins, changing its color. The test measures how much dye remains unbound, which reflects protein concentration. This method is sometimes used historically or in specific clinical contexts to evaluate protein status or liver function.

Why It’s Done

Doctors may order this test if they suspect liver disease, protein malnutrition, or other conditions affecting protein levels. It can also be part of broader liver function testing, though modern tests like albumin or bilirubin are more commonly used.

Common Uses and Limitations

While BCG dye binding was historically used to assess protein levels, it is now less common due to more precise and widely available tests. This test may still appear in older lab reports or specific research settings.

What to Expect Before the Test

No special preparation is needed for this test. You’ll provide a blood sample, typically drawn from your arm. If you’re concerned about pain or discomfort, let your healthcare provider know.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

BCG Dye Binding Test

Frequently asked questions

How long does it take to get results from this test?
Results for this test are usually processed quickly, often within a few hours to a day, depending on lab workflow. However, since this method is less common, results may be reviewed by a specialist if needed.
Can this test detect liver disease?
This test can indirectly reflect protein levels, which may be affected in liver disease, but it is not a primary diagnostic tool. More specific liver function tests are typically preferred for this purpose.
Is there any risk or side effect from this blood draw?
Like all blood draws, there is a small risk of bruising, swelling, or discomfort at the site. Rarely, a more serious reaction like fainting or excessive bleeding may occur. Your healthcare provider will guide you on precautions if you have any concerns.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy