Protein Electrophoresis (Serum) with Immunofixation
This test measures the different types of proteins in your blood, including albumin and globulins, and checks for any unusual proteins that may indicate conditions like multiple myeloma or other disorders affecting protein production.
["Helps identify protein abnormalities that may signal underlying health issues."]
Key facts at a glance
- LOINC
- 80348-6
- Short code
- EPEC eae gene Stl Ql NAA+n-prb
- Synonyms
- EPEC eae gene Stl Ql NAA+n-prb
- Category
- chemistry
- Sample
- stool
- Container
- Clean container
- Units
- N/A
- Ref low
- 0
- Ref high
- 5
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- non-probe-amp-tar
- CPT
- 82378
- SNOMED
- 394995008
- Specimen volume
- 5 g
- Stability
- Refrigerated: 24h, Frozen: 7d
- TAT
- 4–24 hours
- Order
- routine
What Proteins Are Measured?
The test separates blood proteins into bands based on size and charge. Key proteins include albumin (the most abundant), globulins (which include antibodies and other immune proteins), and smaller proteins like alpha-1 and alpha-2 globulins. Immunofixation further identifies specific proteins that may be abnormal, such as monoclonal proteins in conditions like multiple myeloma.
Why Is This Test Done?
Doctors may order this test to diagnose or monitor conditions like multiple myeloma, amyloidosis, chronic inflammatory diseases, or kidney disease. It can also help evaluate symptoms such as fatigue, weight loss, or unexplained protein in urine.
Sample Collection and Preparation
A blood sample is drawn from a vein, usually in your arm. The blood is then processed to separate serum (the liquid part after clotting). The serum is analyzed using electrophoresis to separate proteins and detect any unusual patterns or proteins.
What to Expect Before the Test
No special preparation is typically needed, but you may be asked to fast for 8–12 hours before the test if instructed by your healthcare provider. Wear comfortable clothing and bring any necessary medications.
Reference range
Non-smokers: <2.5 ng/mL; Smokers: <5.0 ng/mLN/A
No special preparation required. For PSA, avoid sexual activity for 24-48 hours before the test.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Abnormal protein patterns can indicate conditions such as multiple myeloma (where abnormal proteins called monoclonal gammas appear), kidney disease (where protein loss may be detected), or chronic infections or autoimmune diseases. High or low levels of specific proteins can also signal other health concerns that require further evaluation.
Short name
Serum Protein Electrophoresis
Frequently asked questions
What does a high or low protein level mean?
How long does it take to get results?
Can I eat or drink before this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy